Displaying items by tag: ChromaTOF

I use LECO PEGASUS® BT 4D to separate, identify and quantify secondary metabolites of various organisms. The advantages of the GC×GC system in separation of multi-component mixtures allow us to separate and identify a huge number of compounds in every samples. The ability to separate and quantify low levels of target analytes in mixtures simultaneously with the major components presented at a high level of concentration and to separate coeluted compounds is a great advantage of two-dimensional GC in comparison with one-dimensional GC. The user-friendly ChromaTOF software and excellent technical support make LECO a reliable partner for researcher. Since we started working on this equipment, our results have become more accurate and comprehensive, and our research has reached a new level.

We started using the LECO PEGASUS® BT GC-TOF mainly for target analysis of aliphatic hydrocarbons on environmental matrices; over time we had the need to develop a method for the qualitative characterization of hop leaves samples for a research project, the ChromaTOF® deconvolution software allowed the identification of several compounds, in particular different terpenes. The Pegasus BT GC-TOF thus proved to be an excellent system, easy to use, with high sensitivity and reproducibility, able to perform both non target and target analysis.

I started working with the PEGASUS GC-HRT+ 4D back in my student years. As a novice researcher, with no experience working with similar devices and no training, it was not easy at first. However, after years of working with this instrument and when compared with other sophisticated equipment in the field of gas chromatography - high-resolution mass spectrometry, I can confidently say that the ChromaTOF is the most user-friendly software of all those available now. Such a variety of functions, as well as very clear, convenient, and fast operation, combined with high-tech equipment, allows to get very decent results.

Over the past 5 years of using the Pegasus GC-HRT+ 4D, we have been able to conduct many various environmental studies, identifying trace amounts of substances in the cleanest Arctic environmental objects. At the same time, such a powerful combination of two-dimensional gas chromatography with time-of-flight mass spectrometry made it possible to analyze complex matrices, namely peat combustion products, herbal and tobacco cigarette smoke, petroleum and much more.

It is worth noting the work of the support service, which helped us very quickly and efficiently. I would like to thank the engineers for their work, who were on call almost 24/7. There are still many projects and tasks ahead that will undoubtedly be solved with the help of the Pegasus GC-HRT+ 4D.

"I use a LECO mass spectrometer for plant metabolomics ; it is an efficient machine with a very low detection limit and the Chromatof deconvolution algorithm allows to find hundreds of compounds in complex extracts."

"Compared to GC equipped with a single column, the Pegasus 4D enabled me to separate unbelievable number of analytes! Tens of thousands of separated peaks in one run was not an exception. But where the separation was not perfect, ChromaTOF software and its deconvolution algorithm did the job. Such excellent ‘separating power’ helped me discover new types of plant PCB metabolites."